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dBBQs : dataBase of Bacterial Quality scores

By March 21, 2017Posters

Visanu Wanchai, Preecha Patumcharoenpol, Intawat Nookaew, and David Ussery

Background: It is well-known that genome sequencing technologies are becoming significantly cheaper and faster. As a result of this, the exponential growth in sequencing data in public databases allows us to explore ever growing large collections of genome sequences. However, it is less known that the majority of available sequenced genome sequences in public databases are not complete, drafts of varying qualities. We have calculated quality scores for more than 100,000 bacterial genomes from all major genome repositories and put them in a fast and easy- to-use database.

Results: Prokaryotic genomic data from all sources were collected and combined to make a non- redundant set of bacterial and archaeal genomes. The genome quality score for each was calculated by four different measurements: assembly quality, number of rRNA and tRNA genes, and the occurrence of conserved functional domains. The dataBase of Bacterial Quality scores (dBBQs) was designed to store and retrieve quality scores. It offers searching function with Elasticsearch, a fast and scalable search and analytics engine for large scale database. In addition, the search results are shown in interactive JavaScript charts using dc.js. The analysis of quality scores across major public genome databases find that most (perhaps 80% or more) of the genomes are of acceptable quality for many uses. However, some genome sequences are of very quality, in a few cases even for ‘complete’ genomes.

Conclusion: dBBQs provides genome quality scores for all available prokaryotic genome sequences with a user-friendly Web- interface. These scores can be used as cut-offs to get a high- quality set of genomes for testing bioinformatics tools or improving the analysis. Moreover, all data of the four measurements that were combined to make the quality score for each genome, which can potentially be used for further analysis. dBBQs will be updated regularly as number of genomes in public databases growing rapidly and is freely use for non-commercial purpose. This work is funded in part from the Arkansas Research Alliance and the Helen Adams & Arkansas Research Alliance Professor & Chair.

Arkansas Center for Genomic Epidemiology & Medicine and The Department of Biomedical Informatics, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205

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