An Assessment of Oxford Nanopore Sequencing for Human Gut Metagenome Profiling: A Pilot Study of Head and Neck Cancer Patients

By October 15, 2019Publications

Thidathip Wongsurawat, Mayumi Nakagawa, Omar Atiq, Hannah N. Coleman, Piroon Jenjaroenpun, James I. Allred, Angela Trammel, Pantakan Puengrang, David W. Ussery, Intawat Nookaew

J Microbiol Methods. 2019 Oct 15:105739. doi: 10.1016/j.mimet.2019.105739. PMID: 31626891

Abstract

Gut metagenome profiling using the Oxford Nanopore Technology (ONT) sequencer was assessed in a pilot-sized study of 10 subjects. The taxonomic abundance of gut microbiota derived from ONT was comparable with Illumina Technology (IT) for the high-abundance species. IT better detected low-abundance species through amplification, when material was limited.

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